Comparison of diethylnitrosamine-induced primary liver cancer models in mice at different developmental stages
SHI Qianqian
RU Mengyao
BAI Yu
ZHANG Kun
GUO Yan
BAI Zhantao
SHEN Lan
Abstract：Objective To systematically compare the differences in tumorigenic efficiency,pathological characteristics,and molecular phenotypes between neonatal(14 d post-birth)and adult(7 weeks old)C57BL/6 mouse models of diethylnitrosamine-induced hepatocellular carcinoma(HCC).Methods Thirty wild-type C57BL/6 mice were selected and divided into a normal group,a neonatal group,and an adult group,with 10 mice in each group.The induced HCC mouse model was established by intraperitoneal injection of carbon tetrachloride combined with a high-fat diet.The survival rates,liver histomorphology,serum liver function indicators[alpha-fetoprotein(AFP),alanine aminotransferase,aspartate aminotransferase,total bilirubin,direct bilirubin,albumin,and triglyceride],pathological staining(HE,PAS,and ORO),and immunohistochemical staining were dynamically monitored to observe the expression and distribution of Ki67 and AFP.Results The pathological features of liver tissues and serum AFP levels(P<0.01)in the neonatal group more closely resembled the clinical progression pattern of HCC.In contrast,the adult group exhibited a prolonged latency period and milder metabolic disturbances.Conclusion This study provides critical experimental evidence for the selection of induced HCC animal models and advances research on developmental stage-dependent carcinogenic mechanisms.
Keywords：liver neoplasmsdiethylnitrosaminedisease modelsanimalage factorscarcinogenesisimmunohistochemistrydiethigh-fatneoplasm grading
Publication Date：2026-04-30
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 565-570 )