Effects of lncRNA RUSC1-AS1 on the growth and epithelial-mesenchymal transition of human hepatocellular carcinoma cell SK-HEP-1 xenograft in nude mice
SONG Yongshun
HU Jun
GAO Lixia
TANG Xiaoqian
JIAN Huiling
Abstract：Objective To investigate the effects of lncRNA RUSC1-AS1 on the growth and epithelial-mesenchymal transition of human hepatocellular carcinoma cell SK-HEP-1 xenograft in nude mice,and to explore its mechanism.Methods RUSC1-AS1 shRNA and negative control recombinant plasmids were constructed.The model of human hepatocellular carcinoma cell SK-HEP-1 xenograft was established in nude mice.The nude mice were divided into three groups:Control group,shRNA group and shRNA-NC group.Mice in shRNA group and shRNA-NC group were injected with plasmid through tail vein.The body mass of mice was observed and the tumor volume was recorded during plasmid injection.The tumor tissues were collected and photographed after the last injection.HE staining was used to observe the pathological status of tumor tissues in each group.The mRNA and protein expression levels of E-cadherin(E-cad),N-cadherin(N-cad)and Vimentin in tumor tissues of each group were detected by qRT-PCR and Western blotting.The expression of RUSC1-AS1 and miR-125b-5p in tumor tissues of each group was detected by qRT-PCR.Results Compared with Control group or shRNA-NC group,body mass and mRNA and protein expression levels of E-cad in tumor tissues of shRNA group were significantly increased(P＜0.05).Tumor volume and mRNA and protein expression levels of N-cad and Vimentin in tumor tissues were significantly decreased(P＜0.05).The expression of miR-125b-5p in tumor tissues of nude mice in shRNA group was significantly increased(P＜0.05).Conclusion Interference with the expression of RUSC1-AS1 can significantly inhibit the growth and epithelial-mesenchymal transition of human hepatocellular carcinoma cell SK-HEP-1 xenograft in nude mice,and its mechanism may be related to upregulating the expression of miR-125b-5p.
Keywords：hepatocellular carcinomaRUSC1-AS1nude micexenograftepithelial-mesenchymal transitionmiR-125b-5p
Publication Date：2025-12-31
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 1686-1691 )