Therapeutic effects of T7 peptide-modified ultra-small polydopamine nanoparticles on traumatic brain injury
QIN Jiale
YI Chunlin
LI Han
CUI Hao
GONG Wenshuai
WU Hongyan
ZHOU Siyuan
LIU Daozhou
Abstract：Objective To fabricate T7 peptide-modified ultra-small polydopamine nanoparticles(T7-UPDA)with the ability to target brain capillary endothelial cells bEnd3 and BV2 microglia,efficiently scavenge reactive oxygen species(ROS),and promote the polarization of BV2 cells toward the M2 phenotype,thereby offering a novel therapeutic strategy for traumatic brain injury(TBI).Methods The in vitro biocompatibility of T7-UPDA was evaluated through hemolysis assays and cytotoxicity experiments.The particle size,zeta potential,and stability of T7-UPDA were characterized using a laser particle size analyzer.The uptake efficiency of T7-UPDA by bEnd3 cells and scratched BV2 cells,its ability to scavenge intracellular ROS in scratched BV2 cells,and its effect on BV2 cell polarization were detected via flow cytometry.Results T7-UPDA exhibited a particle size of approximately(35.1±0.8)nm with a spherical nanoparticle morphology,and no significant change in particle size was observed in 7 d.T7-UPDA demonstrated good biocompatibility in vitro.It effectively targeted both bEnd3 cells and scratched BV2 cells,efficiently scavenged ROS in scratched BV2 cells,and promoted the polarization of BV2 cells toward the M2 phenotype.Conclusion T7-UDPA exhibits the ability to target bEnd3 cells and scratched BV2 cells.By scavenging ROS and reprogramming the polarization state of microglia,it synergistically exerts antioxidant and anti-inflammatory effects,offering new insights for developing nanotherapeutic strategies against secondary brain injury.
Keywords：ultra-small polydopamine nanoparticlesT7 peptidetraumatic brain injuryreactive oxygen species scavengingBV2 cell polarizationbrain targetingdrug delivery systembiocompatibility
Publication Date：2025-11-30
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：8( 1425-1432 )