Impact of configuration differences of chiral ZnO nanoparticles on its frostbite treatment effect
WANG Chaoli
QIAO Youbei
GAO Hao
SUN Chun
ZHAI Yuan
YANG Changbin
CHEN Peng
Abstract：Objective To investigate the structure-activity relationship between different types of chiral ZnO nanoparticles(nano-ZnO)and frostbite treatment.Methods Using zinc acetate as the zinc source and glutamic acid(Glu),valine(Val),and tyrosine(Tyr)with different chiral structures as chiral inducers,chiral nano-ZnO was prepared via the co-precipitation method.Dynamic light scattering was employed to characterize the particle size and surface charge of chiral nano-ZnO.Transmission electron microscopy was used to observe the morphology of chiral nano-ZnO.The chirality of the material was investigated by circular dichroism spectroscopy.The reactive oxygen species scavenging effects of different chiral ZnO was examined by 1,3-diphenylisobenzofuran probe.A mouse frostbite animal model was established to evaluate the frostbite therapeutic effect of chiral nano-ZnO with different chiral structures.Additionally,anti-oxidative stress capacity of chiral nano-ZnO with different chiral structures was compared with an oxygen-glucose deprivation/reperfusion(OGD/R)cell model.Results The D-Tyr-ZnO,L-Val-ZnO,L-Tyr-ZnO,D-Val-ZnO,L-Glu-ZnO,and D-Glu-ZnO constructed by the co-precipitation method had chiral structures.Different chiral designs had a significant impact on the particle size,surface charge,and antioxidant capacity of nano-ZnO.There were notable differences in the therapeutic effects of chiral nano-ZnO with different chiral structures in frostbite animal model,especially L-Glu-ZnO showed better efficacy than those with other chiral structures(P＜0.05).In the OGD/R cell model,L-Glu-ZnO could significantly inhibit the cellular oxidative stress level(P＜0.05).Conclusion The chiral structure exerts an influence on the frostbite treatment efficacy of nano-ZnO.L-Glu-ZnO demonstrates promising potential for clinical applications.
Keywords：zinc oxidechiralityfrostbitenanomedicineanti-oxidative stressamino acidsstructure-activity relationshipL-Glu-ZnO
Publication Date：2025-11-30
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 1415-1420 )