Study on the relationship between serum expression of miR-145-5p and miR-150-5p and pathological characteristics and prognosis in patients with chronic obstructive pulmonary disease combined with non-small cell lung cancer

HE Ting; LI Yuling; XIONG Junfang

doi:10.13276/j.issn.2097-1656.2025.10.015

Abstract：Objective To study the relationship between serum miR-145-5p and miR-150-5p expression and pathological characteristics and prognosis in patients with chronic obstructive pulmonary disease(COPD)combined with non-small cell lung cancer(NSCLC).Methods A total of 126 patients with COPD combined with NSCLC admitted to Lishui Hospital of Wenzhou Medical University from December 2016 to December 2020 were selected as the study group,and 100 patients with simple COPD admitted during the same period were selected as the control group.qRT-PCR was used to detect and compare the relative expression levels of serum miR-145-5p and miR-150-5p in the two groups.The relationship between serum miR-145-5p and miR-150-5p expression and pathological characteristics of patients with COPD combined with NSCLC was analyzed.Patients with COPD combined with NSCLC were followed up for 3 years after surgery.The 3-year overall survival rate was calculated,and the Kaplan-Meier survival curves were plotted to analyze and compare the overall survival rates at 3 years in the low/high expression groups of serum miR-145-5p and miR-150-5p.Results When compared with the control group,the expression of miR-150-5p was significantly upregulated in serum samples of the study group(P＜0.001),while the expression of miR-145-5p was significantly downregulated in serum samples(P＜0.001).Compared with patients without lymph node metastasis and TNM stage Ⅰ-Ⅱ,the proportion of patients with low expression of serum miR-145-5p and high expression of miR-150-5p was higher in patients with lymph node metastasis and TNM stage Ⅲ(P＜0.05).After 3 years of follow-up,among 126 NSCLC patients,4 were lost to follow-up,with an overall survival rate of 36.07％(44/122).In terms of 3-year overall survival rate,the group with higher miR-145-5p expression levels(45.95％)was significantly better than the group with lower expression levels(31.76％)(P＜0.05).On the contrary,the 3-year overall survival rate of the miR-150-5p high expression group(30.00％)was significantly lower than that of the low expression group(47.62％),and this difference was statistically significant(P＜0.05).Conclusion The combination of COPD and NSCLC leads to low expression of serum miR-145-5p and high expression of miR-150-5p,and the expression of serum miR-145-5p and miR-150-5p is associated with lymph node metastasis,increased TNM stage and decreased 3-year overall survival rate,which is expected to become a new molecular biomarker for diagnosis and treatment of COPD combined with NSCLC.