Establishment and phenotypic analysis of oxidative stress-induced dental pulp cell senescence and animal aging models
ZHANG Chongyang
HE Yani
LIU Han
HUI Jun
CHEN Hongwei
JIANG Wenkai
YUAN Yifang
WANG Shengchao
Abstract：Objective To compare and analyze the differences between oxidative stress-induced dental pulp cell senescence and animal aging models and natural aging.Methods The optimal H2O2 stimulation concentration for inducing senescence in human dental pulp fibroblasts was determined via CCK-8 assays and senescence-associated beta-galactosidase staining.Cell proliferation ability and expression of senescence-associated secretory phenotype(SASP)genes were analyzed using flow cytometry,EdU staining,γ-H2AX immunofluorescence staining,and qRT-PCR.A mouse model of aging was established through subcutaneous administration of D-galactose,followed by detection of organ indices and aging-associated biochemical markers.Results Exposure of cells to 200 μmol/L H2O2 for 1 h induced proliferation ability decline,cell cycle arrest,DNA damage,and upregulation of SASP genes,mirroring replicative senescence(P＜0.01,P＜0.05).Subcutaneous D-galactose administration[250 mg/(kg·d)]in mice decreased organ indices,altered oxidative stress-associated biochemical markers,and elevated serum IL-1β and TNF-α(P＜0.01,P＜0.05),consistent with the features of natural aging.Conclusion The aging phenotypes of H2O2-stimulated cellular senescence model and D-galactose-induced mouse aging model are similar to natural aging,providing model tools for the research on the mechanism and prevention of senescence.
Keywords：cellular senescenceoxidative stresshydrogen peroxideD-galactosedental pulpanimal modelssenescence-associated secretory phenotypereplicative senescence
Publication Date：2025-10-31
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：7( 1268-1274 )