Effects of puerarin combined with pulsed electromagnetic fields on mineralization and maturation of osteoblasts
WEI Zhenlong
GAO Yuhai
SHEN Dongshuai
ZHANG Mei
LI Xing
LI Yuanchang
CHEN Keming
Abstract：Objective To explore the effect of puerarin(Pue)combined with low frequency pulsed electromagnetic fields(LFPEMFs)on the mineralization and maturation of rat osteoblasts.Methods The cultured neonatal rat skull osteoblasts were divided into four groups:control(Ctrl)group,Pue group,pulsed electromagnetic fields(PEMFs)group and Pue combined with pulsed electromagnetic fields(Pue+PEMFs)group.Total RNA was extracted after Pue combined with PEMFs for 2 d,and the expression levels of alkaline phosphatase(ALP),Runt-related transcription factor 2(RUNX2),osterix(OSX)and bone morphogenetic protein 2(BMP2)were detected by qRT-PCR.The expressions of type Ⅰ collagen(COL Ⅰ),OSX,RUNX2,osteoprotegerin(OPG),and BMP2 were detected by Western blotting.Pue combined with PEMFs was treated for 3 d,and ALP activity was detected after 6 d.Alizarin red staining was performed after 14 d.Results After Pue combined with PEMFs treatment for 2 d,compared with Ctrl group,the gene expressions of ALP,RUNX2,OSX and BMP2 in Pue+PEMFs group were significantly increased(P＜0.01).The expressions of COL Ⅰ,OSX,RUNX2,OPG and BMP2 were significantly increased(P＜0.01).After 3 and 6 d of Pue combined with PEMFs treatment,compared with Ctrl group,the ALP activity of osteoblasts in Pue+PEMFs group was significantly increased(P＜0.01).The mineralization ability of osteoblasts was significantly improved.Conclusion Based on the results of this study,Pue combined with LFPEMFs can promote the mineralization and maturation of osteoblasts.
Keywords：puerarinelectromagnetic fieldosteoblastalkaline phosphatasecalcified nodulemineralization and maturation
Publication Date：2025-05-31
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 639-644 )