Mechanism of action of Erzhi Pill in treating premature ovarian failure based on network pharmacology and molecular docking
ZHANG Jinyuan
LI Huijuan
CHEN Zhiyong
LI Nan
HE Fengjie
Abstract：Objective To explore the mechanism of Erzhi Pill in the treatment of premature ovarian failure(POF)based on network pharmacology.Methods The active ingredients and targets of Ligustrum lucidum Ait.and Eclipta prostrata(L.)L.were collected through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.The standard protein names were obtained by UniProt and the target gene names were converted.The gene targets of POF and targets of traditional Chinese medicine(TCM)compounds were collected by GeneCards,OMIM,and DrugBank,and their intersection was obtained.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed for potential targets.The TCM component-target-pathway map was constructed.AutoDock Vina software was used to verify the molecular docking between key active ingredients and core targets.Results Seven core targets were obtained by topological analysis,including epidermal growth factor receptor(EGFR),IL-6,tumor protein p53,mitogen-activated protein kinase 14,RAC-alpha serine/threonine-protein kinase,c-Fos protein,and mitogen-activated protein kinase 1.A total of 172 signaling pathways were obtained through KEGG enrichment analysis.Erzhi Pill might have therapeutic effects on POF through IL-17,P13/Akt,EGFR,FoxO,HIF-1 and other signaling pathways.The minimum binding energies of core component-target molecular docking were all less than-24.24 kJ/mol,which indicated that the ligand molecules were well docked with the receptor protein.Conclusion Erzhi Pill can treat POF through multiple target genes and molecular pathways.
Keywords：Erzhi Pillpremature ovarian failurenetwork pharmacologymechanism of actionmolecular docking
Publication Date：2025-04-30
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：7( 493-499 )