Protective effect of peony petal powder on acute ethanol-induced multiple organ injury in mice

LI Yuxin; YANG Xu; XIE Yanhua; WANG Siwang

doi:10.13276/j.issn.2097-1656.2025.04.002

Abstract：Objective To explore the protective effect of peony petals freeze-dried superfine powder(PFSP)on acute ethanol-induced multiple organ injury,and to provide a theoretical basis for further development of PFSP as a protective product for anti-alcohol and ethanol-induced multiple organ injury.Methods A total of 80 mice were randomly divided into 8 groups,including control group,model group,bifendate group,and PFSP groups with different doses,with 10 mice in each group.Mice in control group and model group were gavaged with equal volume of distilled water,and the remaining groups were gavaged with 150 mg/kg bifendate and 20,40,80,160,and 320 mg/kg PFSP,once a day for 10 d.Except for the control group,the remaining groups were gavaged with 15 mL/kg 560 mL/L ethanol to establish a mouse model of acute liver injury 30 min after the last administration,and blood was taken from the eyeballs and organs after 8 h.The activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in serum were detected by biochemical methods.The activities of triglyceride(TG),malondialdehyde(MDA),superoxide dismutase(SOD)and alcohol dehydrogenase(ADH)in liver tissue homogenates,glutathione peroxidase(GSH-Px),catalase(CAT)in gastric tissue homogenates,creatine kinase(CK),lactate dehydrogenase(LDH)in cardiac tissue homogenates,and glutathione(GSH),nitric oxide(NO)in brain tissue homogenates were detected.The histopathologic observation of liver,stomach,heart and brain was carried out on mice.Results The levels of AST and ALT in the serum of mice in PFSP groups were significantly reduced and showed a certain dose effect(P＜0.01),that is,the content decreased more significantly with increasing dose.The liver and stomach coefficients were basically at normal levels.The content of TG and MDA in liver tissue were significantly lower than those in the model group(P＜0.05),while the activity of ADH and the content of SOD were significantly higher than those in the model group(P＜0.05).Except for the PFSP group of 20 mg/kg,the content of GSH-Px in gastric tissue significantly increased and showed a certain dose effect(P＜0.01),while the content of CAT in gastric tissue increased without significant differences.The activity of CK and LDH in heart tissue decreased(P＜0.05).The content of GSH in brain tissue increased,while the content of NO decreased without significant differences.The observation of pathological sections of liver,stomach,heart,brain and other tissues confirmed that PFSP groups showed significant changes in edema and inflammation compared to the model group.Conclusion PFSP has a significant preventive and therapeutic effect on acute liver and stomach injury caused by ethanol in mice.