A series of case studies on the treatment of bone defects in children with enriched stem cell-loaded artificial bone
LIU Xiang
WANG Guoliang
WANG Hu
LIU Yunyan
LI Chenyu
WANG Chaoli
ZHANG Yunfei
Abstract：Objective To investigate the clinical efficacy of immediate intraoperative enrichment of stem cells combined with calcium sulfate-calcium phosphate composite artificial bone in the treatment of bone defects in children.Methods A retrospective analysis was conducted on 11 pediatric patients with bone defects admitted to the Department of Orthopaedics,Tangdu Hospital,Air Force Medical University from July 2015 to July 2024.The feasibility and advantages of integrating autologous bone marrow mesenchymal stem cells(BMSCs)into calcium sulfate-calcium phosphate composite artificial bone during surgery were analyzed.This was achieved through a screen-enrich-combine circulating system implemented intraoperatively.The immediate enrichment effect of BMSCs in calcium sulfate-calcium phosphate composite artificial bone particles and its repair effect on bone defects in children were analyzed.Bone healing was assessed utilizing Samantha X-ray scoring system.Additionally,the adjacent joint function and quality of life before and after surgery were evaluated by Paley score and SF-36 score.Results In vitro experiments demonstrated favorable biocompatibility of the artificial bone,with optimal cell viability after coculture with human BMSCs for an extended period.Autologous BMSCs exhibited high enrichment efficiency on the surface of calcium sulfate-calcium phosphate artificial bone particles,indicating its efficacy in the treatment of bone defects in children.A total of 11 pediatric patients were enrolled in this study,with a follow-up duration of(16.3±5.8)weeks.Postoperative observations revealed no complications such as infection,bleeding,repair failure,intractable pain,and fracture.The repair outcome of bone defects was deemed satisfactory.Conclusion Immediate intraoperative enrichment of autologous BMSCs into calcium sulfate-calcium phosphate composite artificial bone represents a mature clinical approach,which stands as a satisfactory and feasible method for bone defect repair in children.
Keywords：childrenbone defectstem cell enrichmentcalcium sulfate-calcium phosphate composite
Publication Date：2025-03-31
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：8( 378-385 )