Short-peptide-based enteral nutrition improves the prognosis of nerve function in mice with traumatic brain injury by protecting intestinal mucosal barrier function
WANG Kai
ZHANG Ziwen
WANG Tinghao
WANG Jiahui
GE Shunnan
QU Yan
Abstract：Objective To investigate the protective effect of short-peptide-based enteral nutrition(SPEN)on intestinal mucosal barrier function and its effect on secondary brain injury in mice with traumatic brain injury(TBI).Methods Male clean-grade C57BL/6 mice aged 8 to 12 weeks were divided into Sham group,TBI group and TBI+SPEN group.Mice in the TBI+SPEN group were given short-peptide powder(0.4 g/L)in drinking water immediately after modeling,while the other groups received sterile water during the same process.Firstly,behavioral changes of the three groups of mice were analyzed to determine the effect of SPEN on the prognosis of nerve function in TBI mice.Secondly,the changes of the structure and permeability in gut and the level of lipopolysaccharide(LPS)in blood were analyzed after TBI mice were given SPEN.In addition,the water content of brain tissues,the level of inflammatory factors and the activation of microglia were detected to determine the effect of SPEN on secondary brain injury after TBI.Results Compared with Sham group,the intestinal mucosal barrier structure was damaged,the permeability was increased,and the entry of LPS into blood and brain was increased in TBI group(P＜0.01).However,the damage could be effectively reversed in TBI+SPEN group.At the same time,the activation of microglia and the expression of inflammatory factors in brain tissue were reduced,and the neurological function was significantly improved.Conclusion Early SPEN can protect intestinal mucosal barrier function,reduce the entry of LPS into blood and brain,alleviate secondary brain injury and improve the prognosis of TBI mice.
Keywords：traumatic brain injuryintestinal barriershort-peptide-based enteral nutritionendotoxin
Publication Date：2025-03-31
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 318-323 )