Effect of transplantation of adipose tissue-derived stromal vascular fraction on myocardial ischemia-reperfusion injury in rats
ZHAO Ran
ZHAO Jieqiong
HU Jianqiang
QI Bingchao
LI Yan
Abstract：Objective To investigate the therapeutic effect of intravenous transplantation of adipose tissue-derived stromal vascular fraction(SVF)on myocardial ischemia-reperfusion injury(MIRI)in rats.Methods SVF was isolated and cultured from SD rats by collagenase,and 36 male SD rats were randomly divided into sham-operated group,MIRI group,MIRI+0.3 × 106 SVF/kg(treatment group 1),MIRI+1 × 106 SVF/kg(treatment group 2),MIRI+5 × 106 SVF/kg(treatment group 3)and MIRI+10 × 106 SVF/kg(treatment group 4),with 6 rats in each group.MIRI model was constructed in all groups except the sham-operated group.Echocardiography was used to detect the cardiac function of rats,TTC staining was used to observe the area of myocardial infarction,HE staining was used to observe the pathological damage of myocardium,Masson staining was used to observe myocardial fibrosis,TUNEL staining was used to observe myocardial apoptosis,immunohistochemistry was used to observe CD31 expression in myocardial tissue,and Western blotting was used to detect the expression of TGF-β1 in myocardial tissues of rats.Results Compared with the sham-operated group,the area of myocardial infarction was significantly increased in MIRI group,the cardiac systolic function was markedly reduced(P＜0.01),the degrees of cardiomyocyte disarrangement and myocardial fibrosis were observably increased(P＜0.01),the rate of cardiomyocyte apoptosis was significantly increased,the expression of CD31 was decreased,and the expression of TGF-β1 was notably increased(P＜0.01).Compared with the MIRI group,the cardiac systolic function was significantly improved in the SVF treatment group(P＜0.01),the degrees of cardiomyocyte disarrangement and myocardial fibrosis were significantly decreased(P＜0.01),the rate of cardiomyocyte apoptosis was significantly decreased,the expression of CD31 was significantly increased,and the expression of TGF-β1 was decreased(P＜0.01)in a dose-dependent manner.Conclusion Intravenous transplantation of SVF can reduce the degrees of myocardial fibrosis and cardiomyocyte apoptosis,alleviate myocardial injury and improve cardiac function in rats with myocardial ischemia-reperfusion,and the mechanism may be related to its promotion of myocardial tissue vascular regeneration and inhibition of TGF-β1 expression in myocardial tissue.
Keywords：adipose tissue-derived stromal vascular fractionmyocardial ischemia-reperfusionmyocardial fibrosiscardiomyocyte apoptosisvascular proliferationTGF-β1
Publication Date：2025-02-27
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：7( 167-173 )