Protective effect and mechanism of salvianolate on myocardial infarction in mice with diabetes
YANG Xue
PU Siying
ZHAO Meina
LIANG Qi
GENG Jing
GUO Dong
WANG Ying
HU Lang
LI Yan
Abstract：Objective To investigate the protective effect and mechanism of salvianolate(SAL)on myocardial infarction(MI)in mice with diabetes.Methods Adult male C57BL/6J mice were set as the control group,and spontaneous type 2 diabetic mice(db/db)were divided into model group,positive drug group and SAL administration group(50,100,and 200 mg/kg),with 6 mice in each group.The left anterior descending coronary artery of the mice was ligated to establish an MI injury model.The drug was administered continuously for 7 d.Color Doppler ultrasound was used to evaluate the cardiac function indexes of mice.In addition,HE staining technology was used to detect the pathological damage of myocardial tissue.Von Willebrand factor(vWF),platelet endothelial cell adhesion molecule-1(PECAM-1),E-selectin,nitric oxide(NO),reactive oxygen species(ROS),interleukin-1 β(IL-1β),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and other indicators in mouse serum were determined by ELISA.The mRNA and protein expression levels of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(VEGFR2),phosphatidylinositol 3-kinase(PI3K),and protein kinase B(Akt)in mouse myocardial tissue were measured using qRT-PCR and Western blotting techniques.Results Compared with the control group,the blood glucose and insulin levels in the model group were higher,and the pathological damage of myocardial tissue was more serious,with decreased left ventricular ejection fraction,left ventricular fractional shortening,and NO levels(all P＜0.01),increased left ventricular internal diameter at end-diastole,left ventricular internal diameter at end-systole,vWF,PEC AM-1,E-selectin,ROS,IL-1β,IL-6 and TNF-α(all P＜0.01),and increased mRNA and protein expressions of VEGF and VEGFR2 in stress(both P＜0.01).Compared with the model group,the positive drug group and the SAL administration group treated with different doses could significantly improve the above changes.Conclusion SAL can inhibit the inflammatory process,restore the damaged function of vascular endothelium,regulate VEGF/PI3K/Akt signaling pathway to improve cardiac function,and play a protective role after MI in diabetic mice.
Keywords：diabetic complicationssalvianolatemyocardial infarctionendothelial functionmice
Publication Date：2025-02-27
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：8( 145-152 )