Expression of miR-125b-5p,miR-1207-3p,and miR-802 in prostate cancer tissues and their relationship with clinicopathologic features and prognosis

WU Yuanbo; LONG Huimin

doi:10.13276/j.issn.2097-1656.2024.12.017

Abstract：Objective To analyze the expression levels of miR-125b-5p,miR-1207-3p,and miR-802 in prostate cancer(PCa)tissues,and to explore the relationship between the indicators and pathological parameters and prognosis.Methods A total of 161 PCa patients were selected as the study subjects,and the cases were collected from January 2019 to July 2021 at Ningbo Medical Center Li Huili Hospital.The patients were followed up from the day of diagnosis until July 2024.During the follow-up period,3 cases were lost to follow-up,and 158 cases were finally included for the study.According to the prognosis,they were divided into the poor prognosis group(53 cases)and the good prognosis group(105 cases).Fresh cancer tissues and paracancerous tissue specimens surgically removed from patients with PCa were collected,and the expression of miR-125b-5p,miR-1207-3p,and miR-802 in cancer tissues and paracancerous tissues were compared.The relationship between the expression of miR-125b-5p,miR-1207-3p,and miR-802 in cancer tissues and clinicopathological features of PCa patients was analyzed,and the expression of miR-125b-5p,miR-1207-3p,and miR-802 in cancer tissues of PCa patients with different prognosis were compared.The risk factors for poor prognosis of PCa patients were analyzed by Cox regression,and the poor prognosis group was included in the positive and the good prognosis group was included in the negative.The predictive value of miR-125b-5p,miR-1207-3p,and miR-802 of cancer tissues on the poor prognosis of PCa patients was analyzed.Area under the curve(AUC)was obtained using receiver operating characteristic curve(ROC).Results The expression of miR-125b-5p and miR-802 in cancer tissues of PCa patients was lower than that in paracancerous tissues(P＜0.05),and the expression of miR-1207-3p in cancer tissues was higher than that in paracancer tissues(P＜0.05).The expression of miR-125b-5p and miR-802 in cancer tissues of PCa patients with Gleason score＞7,stage Ⅲ-Ⅳ,and lymph node metastasis was lower than that in those with Gleason score≤7,stage Ⅰ-Ⅱ,and no lymph node metastasis(P＜0.05),while the expression of miR-1207-3p in cancer tissues was higher than that in those with Gleason score≤7,stage Ⅰ-Ⅱ,and no lymph node metastasis(P＜0.05).The expression of miR-125b-5p and miR-802 in cancer tissues of the poor prognosis group was lower than that in the good prognosis group(P＜0.05),and the expression of miR-1207-3p in cancer tissues was higher than that in the good prognosis group(P＜0.05).Cox regression analysis showed that low expression of miR-125b-5p(HR=1.091),high expression of miR-1207-3p(HR=1.099),and low expression of miR-802(HR=1.135)in cancer tissues were associated with poor prognosis of patients with PCa(P＜0.05).ROC analysis showed that the AUC value of the combined detection of miR-125b-5p,miR-1207-3p,and miR-802 in cancer tissues for predicting poor prognosis in patients with PCa was 0.900,which was higher than that of the three single detections(AUC values were 0.689,0.679,and 0.758,respectively,P＜0.05).Conclusion MiR-125b-5p and miR-802 are lowly expressed in PCa tissues,while miR-1207-3p is highly expressed.The three are related to the pathological parameters and prognosis of patients,and the combined detection of the three was more advantageous in predicting the poor prognosis of patients with PCa.