Visualizing the distribution of mitochondrial molecular mRNAs of cerebellar Purkinje cells by RNAscope
LIU Hui
NI Ziwei
LIU Yuxuan
LI Shujiao
WU Feifei
WANG Lu
YANG Yanling
WANG Yayun
Abstract：Objective To investigate the distribution of mitochondria-associated molecule mRNA in cerebellar Purkinje cells.Methods Transgenic technology was used to prepare Pcp2-tdTomato mouse labeled Purkinje cells.The mRNAs of mitochondria-associated molecules Mfn2,Mfn1,Ucp4,Drp1,Ucp2,Mcu and Nclx were detected in situ by RNAscope technique.Results The results of this study showed that Mfn2,Mfn1,Ucp4 and Drp1 were highly expressed in Purkinje cell body(Bin 3＞10％).However,Ucp2,Mcu and Nclx were less expressed.In addition,Mfn2,Mfn1,Ucp4,Drp1,Ucp2,Mcu and Nclx were less expressed in dendrites.Conclusion Current research focuses on developing new and more specific molecules to regulate the activity of Purkinje cells and opening therapeutic windows for Purkinje cells related diseases.The molecule identification of potential drug targets,mechanisms of action,and structural basis of their activity will crucially promote preclinical development.
Keywords：mitochondrial dynamics proteinsRNAscopePurkinje cellcerebellum
Publication Date：2024-12-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：17( 1353-1369 )