A high sensitivity Western blotting method based on atom transfer radical polymerization
ZHANG Guo'an
ZHANG Aihong
ZHAO Lili
ZHANG Yan
YANG Xiaohui
LIU Ying
ZHANG Jingjing
SUN Wenjie
LIU Yingfu
Abstract：Objective To explore a highly sensitive Western blotting method based on gold nanoparticles(AuNPs)and atom transfer radical polymerization(ATRP).Methods AuNPs was attached to polyvinylidene fluoride(PVDF)membranes through ATRP,and the detection sensitivity was improved by the adsorption of small molecules by PVDF membrane.Results The improved PVDF membrane(PVDF-ATRP-AuNPs)increased the interception efficiency of target small molecules by about 80％and significantly reduced the limit of detection.Conclusion The improved method has good stability and excellent sensitivity,and can effectively detect ultra-low concentration small molecule analytes.
Keywords：Western blottingpolyvinylidene fluoride membranesgold nanoparticles
Publication Date：2024-11-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：4( 1284-1287 )