Effect of photobiomodulation on structural biomarkers of spinal cord injury in rats and clinical validation
LUO Liang
ZHANG Jianxin
ZUO Xiaoshuang
ZHANG Zhihao
LIANG Zhuowen
DING Tan
HU Xueyu
WANG Zhe
Abstract：Objective To explore the effect of photobiomodulation(PBM)on structural biomarkers of spinal cord injury(SCI),so as to create the possibility for structural biomarkers of SCI to be used as the efficacy evaluation index of PBM.Methods In animal experiments,rats were divided into Sham group,SCI group,and PBM group,with 9 rats in each group.BBB scores were performed and serum and cerebrospinal fluid were collected before and 1,3 and 7 d after modeling.The concentrations of structural biomarkers in serum and cerebrospinal fluid of rats at each time point in each group(S100β,GFAP,TAU,MMPs,pNF-H,and NSE)were detected by enzyme-linked immunosorbent assay,the change characteristics of these biomarkers and BBB scores of rats under the intervention of PBM were compared and analyzed,and based on these results,clinical verification was carried out.In clinical experiments,according to the inclusion and exclusion criteria,the recruited SCI patients were divided into simple decompression group+light exposure group and simple decompression group according to whether they were treated with PBM or not.ASIA scores were performed before operation in both groups,and serum samples of the two groups were collected before operation and 1,3 and 7 d after operation.The structural biomarkers(S100β,TAU,pNF-H,and GFAP)that were significantly affected by PBM in animal experiments were detected and compared.ASIA scores were performed again at 3 months post-operative follow-up and compared with preoperative results.Results In animal experiments,BBB scores of rats in PBM group were higher than those in SCI group on the 3rd day after modeling,and significantly higher than those in SCI group on the 7th day(P＜0.01).The concentrations of structural biomarkers in serum and cerebrospinal fluid of rats in PBM and SCI groups began to increase after modeling and reached the peak within 1 to 3 d.The concentrations of S100β,GFAP,TAU and pNF-H in serum of rats in PBM group were significantly lower than those in SCI group at 1,3 and 7 d(P＜0.01).The concentrations of S 100β,GFAP,TAU,MMPs,pNF-H and NSE in cerebrospinal fluid of rats in PBM group were significantly lower than those in SCI group at 1,3 and 7 d(P＜0.01).In clinical experiments,the serum concentrations of TAU,pNF-H and GFAP in DP group were significantly lower than those in D group at 3 and 7 d after operation,and the follow-up showed that the ASIA sensory and motor scores in DP group 3 months after operation were significantly higher than those before operation(P＜0.05).Conclusion PBM can reduce the concentrations of structural biomarkers of SCI in serum and cerebrospinal fluid while promoting the recovery of neural function.
Keywords：spinal cord injuryphotobiomodulationserumcerebrospinal fluidbiomarker
Publication Date：2024-08-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：8( 883-890 )