Expression of lncRNA TP53TG1 in dental pulp and its effect on transcription of inflammatory factors in dental pulp stem cells
DAI Zihan
WANG Minghao
WANG Shengchao
HE Wenxi
Abstract：Objective To investigate the expression difference of lncRNA TP53TG1 in healthy and inflamed dental pulp and its effect on the transcription level of inflammatory factors in human dental pulp stem cells(hDPSCs)induced by lipopolysaccharide(LPS).Methods A probe set against lncRNA TP53TG1 was designed and synthesized.RNA fluorescence in situ hybridization was used to detect the expression of lncRNA TP53TG1 in healthy and inflamed dental pulp tissues.hDPSCs were isolated and cultured by tissue enzymatic digestion method,and the cultured hDPSCs were verified for multidirectional differentiation induction and identified for phenotypic characterization by flow cytometry.hDPSCs were divided into siNC group,siNC+LPS group and siTP53TG1+LPS group,which were transfected with control sequence and TP53TG1 siRNA,respectively.Then the siNC+LPS group and siTP53TG1+LPS group were treated with LPS for 24 h.The mRNA expression levels of IL-8,IL-1β3 and TNF-α in each group were detected by qRT-PCR.Results The expression of lncRNA TP53TG1 was observed in healthy dental pulp tissues,mainly distributed in odontoblasts.The expression of lncRNA TP53TG1 was increased in the inflammatory state and was distributed throughout the dental pulp.After LPS treatment,the mRNA expression levels of TNF-α,IL-1β,and IL-8 in hDPSC increased.Down-regulation of lncRNA TP53TG1 further increased the mRNA expression level of TNF-α in hDPSCs.Conclusion LncRNA TP53TG1 may be involved in the regulation of pulpitis and the immune regulation of odontoblast.
Keywords：lncRNA TP53TG1human dental pulp stem cellspulpitistumor necrosis factor-α
Publication Date：2024-07-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：5( 833-837 )