Treatment of intestinal ischemia-reperfusion injury with T.chebula based on the theory of"simultaneous treatment of lung and intestine"
LIU Chenxu
FENG Yingda
WANG Shoujia
YE Wen
MENG Jingru
LI Xiaoqiang
Abstract：Objective To study the therapeutic effect of T.chebula on intestinal ischemia-reperfusion injury(ⅡRI)in mice based on the theory of"simultaneous treatment of lung and intestine",and to preliminarily discuss whether the effect of T.chebule is based on its reduction of systemic inflammatory response in ⅡRI mice.Methods Thirty healthy male C57BL/6J mice aged 8 weeks were randomly divided into 5 groups:Sham group,model(ⅡRI)group,ⅡRI+T.chebula low,medium and high dose groups,with 6 mice in each group.The ⅡRI model was established by clamping the superior mesenteric artery for 45 min and reperfusion for 90 min.After establishing the ⅡRI model,each group was administrated with different doses of T.chebula once a day for 7 d.Sham group was given normal saline by gavage at the same time point after laparotomy without modeling.ⅡRI group was given normal saline by intragastric administration after modeling.The pathological and morphological changes of intestinal and lung tissues were observed by HE staining,the apoptosis of intestinal cells was detected by TUNEL staining,and the levels of TNF-α,IL-1β and IL-6 in serum were detected by ELISA kit.Results Compared with the Sham group,the intestinal tract of mice in the ⅡRI group showed edema,blood stasis and adhesion as a whole,the intestinal transverse section showed structural damage of lamina propria,the subepithelial space of the intestinal mucosal villi tip was significantly widened,and the villi tip was partially shed,while the overall intestinal injury degree was significantly improved after treatment with T.chebula.Compared with the Sham group,Chiu's score was increased in the ⅡRI group(P＜0.01),while Chiu's score was significantly decreased in T.chebula-treated group(P＜0.01).Compared with the Sham group,the number of intestinal cell apoptosis in the ⅡRI group was significantly increased,while the number of intestinal cell apoptosis in T.chebula-treated group was reduced.Compared with the Sham group,HE results of the ⅡRI group showed obvious thickening of alveolar wall and infiltration of a large number of inflammatory cells in the alveolar space,while the lung injury was significantly improved in T.chebula-treated group.Compared with the Sham group,the lung injury score of mice in the ⅡRI group was significantly increased(P＜0.01),while the lung injury score was decreased after administration of T.chebula,and the difference was statistically significant(P＜0.05,P＜0.01).Compared with the Sham group,lung wet/dry weight ratio in the ⅡRI group was increased(P＜0.05),while that in T.chebula-treated group was decreased,and the degree of pulmonary edema was significantly improved.Compared with the Sham group,the serum levels of pro-inflammatory factors TNF-α,IL-lβ and IL-6 in the ⅡRI group were significantly increased(P＜0.01),while the serum levels of TNF-α,IL-1β and IL-6 in T.chebula-treated group were decreased in a dose-dependent manner,and the difference was statistically significant(P＜0.01).Conclusion T.chebula can effectively alleviate ⅡRI in mice,inhibit intestinal cell apoptosis,and reduce lung injury caused by intestinal ischemia-reperfusion in mice.It plays the role of simultaneous treatment of lung and intestine,which is related to reducing the serum levels of TNF-α,IL-1β and IL-6 and alleviating inflammatory responses in mice.
Keywords：T.chebulaintestinal ischemia-reperfusion injurysimultaneous treatment of lung and intestine
Publication Date：2024-07-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 827-832 )