Expression characteristics of miR-873-5p in colorectal cancer and its correlation with patient prognosis
ZHAO Yihan
SONG Liang
HU Wengting
DENG Jia
YANG Tao
Abstract：Objective To explore the expression characteristics of miR-873-5p in colorectal cancer(CRC)and its correlation with patient prognosis.Methods Target mRNA was screened based on TarBase v.7.0 database.Differentially expressed genes were screened using GEO database,and KEGG signaling pathway enrichment analysis was performed.MiR-873-5p was overexpressed in colon cancer SW480 cells for functional testing.The expression level of miR-873-5p was determined by qRT-PCR.Cell proliferation detection was detected by CCK-8 method.Cell migration was measured by Transwell method.qRT-PCR and Western blotting were used to detect mRNA and protein expression levels in SW-620,LOVO,SW480 cells and normal colon epithelial NCM-460 cells.Flow cytometry was used to detect the apoptosis rate of SW480 cells.High-content screening was used to detect mitochondrial membrane potential in SW480 cells.Results MiR-873-5p was underexpressed in CRC(P＜0.01),while overexpression of miR-873-5p significantly increased the expression of downstream molecules SF1 and NF-κB(P＜0.01),and significantly reduced the proliferation,migration,and apoptosis ability of tumor cells(P＜0.01).KEGG pathway enrichment analysis showed that the downstream pathway of miR-873-5p was enriched in NF-κB pathway,and prognostic analysis showed that the target molecules of miR-873-5p,SF1 and NF-κB,were positively correlated with patients(P＜0.01).Conclusion MiR-873-5p is underexpressed in CRC,while overexpression of miR-873-5p can inhibit the proliferation and migration of colon cancer SW480 cells,and induce cell apoptosis,thus becoming a potential molecular target for CRC.
Keywords：colorectal cancermiR-873-5pdifferentially expressed genesSW480 cells
Publication Date：2024-07-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 775-780 )