Construction and evaluation of C57 mouse model of noise-induced hidden hearing loss in military aviation

JIANG Yihong; ZHANG Min; ZHU Jing; WANG Fei; ZHENG Zeyu; LIU Yuhui; LI Congcong; ZHANG Zhuoru; CHANG Tong; WANG Xiaocheng

doi:10.13276/j.issn.2097-1656.2024.02.008

Abstract：Objective To establish a C57 mouse model of noise-induced hidden hearing loss(HHL)in military aviation and evaluate its effectiveness,so as to provide a stable mouse model for the study of noise-induced HHL in military aviation.Methods Male C57BL/6J mice with normal hearing were randomly divided into 4 groups and exposed to noise at different intensities:105 dB 2 h,110 dB 2 h,110 dB 4 h,and 115 dB 4 h.The mice before noise exposure were taken as the control group.According to different times after noise exposure,they were divided into 1 d after noise exposure(NE-1 d),7 d after noise exposure(NE-7 d),14 d after noise exposure(NE-14 d),and 28 d after noise exposure(NE-28 d).Each experimental group was exposed to military helicopter noise at corresponding intensity,and auditory brainstem response(ABR)was tested at each time node.After screening out the eligible noise exposure intensity,the basement membrane was stained by immunofluorescence and the number of ribbon synapses was verified after the noise exposure.Results After 105 dB 2 h exposure to military helicopter noise,the hearing threshold shift of C57 mice was not significant.After exposure to 110 dB 4 h and 115 dB 4 h of military helicopter noise,the hearing threshold of C57 mice shifted permanently.After 110 dB 2 h exposure to military helicopter noise,the hearing threshold of C57 mice shifted temporarily,the amplitude of ABR wave I decreased,and the number of ribbon synapses decreased,meeting the functional and morphological requirements of HHL.Conclusion Exposure to 110 dB military helicopter noise for 2 h can be used as an ideal stimulus parameter for C57 mouse model of noise-induced HHL in military aviation.