Summary and analysis of perioperative strategies for preventing and treating recipient infections in pig-to-rhesus heart xenotransplantation
ZHOU Yenong
ZHANG Bing
CHEN Tao
YANG Xiuling
JIN Yan
LYU Xiangni
GUO Hong
YI Wei
ZHAO Rong
DUAN Weixun
LIU Jincheng
JIN Zhenxiao
Abstract：Objective To summarize and analyze the perioperative recipient infection and treatment strategies according to the gene-edited pig-to-rhesus heart xenotransplantation in 2022,so as to provide guidance for perioperative infection control of xenotransplantation.Methods The experiment utilized male gene-edited(GTKO/β4GalNT2 KO/CMAH KO/hCD55/hCD46/hTBM)pig as donor and male rhesus as recipient.Pig heart was ectopically transplanted into the abdominal cavities of the recipient.Perioperative intensive care was given to monitor infection indicators including signs,blood routine,serum inflammatory factors,chest radiographs,and bacterial cultures(blood,drainage,secretions,and excretions).The use of anti-infective drugs was adjusted based on changes in these indicators.Results The recipient rhesus survived and the transplanted heart maintained systolic function for 20 d,with minimal fluctuations in body temperature and blood routine,no hyperthermia(＞38.5 ℃)or elevated white blood cell counts.On the 9 th day after surgery,chest X-rays revealed patchy shadows resembling infection,which was improved after adjusting antibiotic usage and strengthening lung care.Postoperative inflammatory indicators,such as C-reactive protein and procalcitonin,did not show significant increases.However,on the 13th day after surgery,bacterial cultures from oral secretions,ascites,and gastric juice were positive for Staphylococcus haemolyticus.Conclusion The high-intensity and high-dose use of immunosuppressive drugs leads to an increased incidence of recipient infections following xenotransplantation.Nonetheless,the timely examination and adjustment of treatment strategies can effectively control the progression of these infections.
Keywords：heart xenotransplantationgene-edited pigrhesuspostoperative infection
Publication Date：2024-01-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 26-31 )