Histidine improves ischemia-reperfusion injury in transplanted liver by antioxidant effects
ZHANG Zhigang
GAO Mingshu
LI Jiaying
XU Xiaojun
WEI Yuanyuan
DU Yulu
WANG Nan
HUANG Qichao
Abstract：Objective To explore the protective effect and related mechanisms of supplementing histidine on ischemia-reperfusion injury in transplanted liver.Methods A mouse model of ischemia-reperfusion injury of transplanted liver was established.Thirty male C57BL/6 mice were randomly divided into three groups:Sham group,normal saline treatment group,and histidine treatment group.Hypoxia/reoxygenation was used to simulate ischemia-reperfusion injury in cells,and T-antigen-immortalized human liver epithelial(THLE)cells were divided into three groups:negative control group,normal saline treatment group and histidine treatment group.The detection indicators were as follows:the liver function of mice was detected by the activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST);Western blotting was used to detect the expression of B-cell lymphoma 2(Bcl-2),Bcl-2-associated X(Bax)protein,and cleared caspase-3 protein levels in liver tissue;TUNEL staining was used to detect the proportion of apoptotic cells in liver cells;HE staining was used to detect the pathological structural changes of liver tissue;and reactive oxygen species(ROS)detection kit was used to detect the level of ROS in THLE cells.Results Compared with the normal saline treatment group of the mouse model of ischemia-reperfusion injury in transplanted liver,the histidine treatment group showed a decrease in the activities of serum ALT and AST(P＜0.05),a significant reduction in liver pathological damage,a decrease in Bax expression,and an upregulation of Bcl-2,a significant decrease in Bax/Bcl-2 ratio,and an increase in cleaved caspase-3 protein levels(P＜0.05).In addition,THLE cell tests showed that histidine treatment could reduce the proportion of liver cell apoptosis and the level of ROS in liver cells.Conclusion Histidine can improve ischemia-reperfusion injury in transplanted liver through its antioxidant effects.This study provides new ideas for clinical treatment of liver ischemia-reperfusion injury.
Keywords：liverischemia-reperfusion injuryhistidinereactive oxygen species
Publication Date：2024-01-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：7( 15-21 )