Detection of porcine endogenous retrovirus in combined liver and kidney xenotransplantation
ZHANG Hong
ZHANG Xuan
TAO Kaishan
DOU Kefeng
Abstract：Objective To detect the expression of porcine endogenous retrovirus(PERV)genes in donor and recipient of combined liver and kidney xenotransplantation,hoping to provide a reference for the cross-species transmission of PERV.Methods The tissue samples of 6-gene-edited pig-to-rhesus macaque combined liver and kidney xenotransplantation were selected as research objects.The expressions of PERV coding gene,PERV subtypes,and porcine mitochondrial cytochrome B gene in different tissues of donor and recipient samples during xenotransplantation were detected by RT-PCR and PCR.Results There were PERV coding genes gag and pol,PERV-A and PERV-B subtypes,and porcine mitochondrial cytochrome B gene in different tissues of donor pig,but no PERV-C subtype was detected in the donor tissue samples.At the end of transplantation,no PERV gene sequences were detected in different tissues of the recipient.Conclusion No cross-species transmission of PERV occurred within 14 d of survival in gene-edited pig-to-rhesus macaque combined liver and kidney xenotransplantation.
Keywords：xenotransplantationporcine endogenous retroviruszoonotic diseasescross-species transmission
Publication Date：2024-01-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：4( 5-8 )