Correlation analysis between renal fibrosis and YAP activation levels in patients with diabetic kidney disease
FENG Lan
YANG Xiaojuan
CHEN Yang
LIU Hongbao
LI Ni
Abstract：Objective To explore the correlation between the level of Yes-associated protein(YAP)and estimated glomerular filtration rate(eGFR)in patients with diabetic kidney disease(DKD).Methods A total of 58 patients diagnosed with DKD by renal biopsy in Tangdu Hospital,Air Force Medical University from February 2017 to April 2022 were selected and divided into 3 groups according to the eGFR staging criteria of chronic kidney disease in KDIGO guidelines:DKD stage Ⅰ(n=19),DKD stage Ⅱ(n=18)and DKD stage Ⅲ(n=21),and 8 patients who underwent nephrectomy were selected as control group.The immunofluorescence staining was performed on renal tissues to analyze the expression level of YAP in DKD patients at different stages and its correlation with different clinical indexes and pathological grading.Results There was no significant difference in the expression of YAP between patients with DKD stage Ⅰ and control group.Compared with patients with DKD stage Ⅰ,the expression of YAP in renal tubular epithelial cells in patients with DKD stage Ⅱ was increased(P＜0.01),and it was mainly distributed in the cytoplasm.However,compared with patients with DKD stage Ⅱ,patients with DKD stage Ⅲ showed more YAP expression and nuclear translocation(P＜0.01).Importantly,renal YAP levels showed a DKD stage-dependent and point-to-point increase(P＜0.01).The level of YAP was significantly correlated with eGFR(r=-0.854,P＜0.01),serum creatinine(r=0.849,P＜0.01)and cystatin C(r=0.717,P＜0.01).In addition,the correlation between YAP level in renal tissue and the extent of interstitial fibrosis(r=0.613 vs r=-0.482),tubular atrophy(r=0.633 vs r=-0.542)and interstitial fibrosis and tubular atrophy score(r=0.522 vs r=-0.377)was better than that between YAP level and eGFR level.Conclusion The level of YAP in renal tissue of DKD patients is related to eGFR,and the correlation with tubulointerstitial damage is better than that with eGFR.The histological analysis of YAP may be a complementary method for evaluating renal fibrosis in DKD patients,which needs further study.
Keywords：diabetes mellitusdiabetic kidney diseasechronic kidney diseaserenal fibrosisYes-associated protein
Publication Date：2023-12-28
Online Publishing Date：2026-08-26(First online date of this platform, not the publication date of the document)
Pages：6( 1209-1214 )