Clinical value of contrast-enhanced ultrasound combined with serum miR-9-5p and miR-214-5p in the diagnosis of be-nign and malignant breast nodules
XING Kai
SUN Jingjing
ZHANG Lu
ZHANG Qiang
Abstract：Objective To explore the clinical value of contrast-enhanced ultrasound(CEUS)combined with serum MicroRNA-9-5p(miR-9-5p)and MicroRNA-214-5p(miR-214-5p)in the diagnosis of benign and malignant breast nodules.Methods Totally,121 breast nodule patients who underwent CEUS examination were assigned into benign group(57 cases)and malignant group(64 cases)based on pathological results.Real-time fluorescence quantitative PCR was applied to determine the levels of serum miR-9-5p and miR-214-5p.ROC curve was applied to analyze the diagnostic efficacy of serum miR-9-5p and miR-214-5p levels for malignant breast nodules.Kappa test was applied to analyze the consistency between CEUS and serum miR-9-5p,miR-214-5p alone or in combination in the diagnosis of malignant breast nodules and pathological results.Results The malignant group had a higher number of nodules,higher peak intensity(PI)and higher serum miR-9-5p levels than the be-nign group,and a lower percentage of patients with early/chronological time to top(TTP),mean transit time(MTT),serum miR-214-5p levels and time to regression than the benign group.In the malignant group,CEUS was characterized by high en-hancement,unclear enhancement borders,irregular enhancement shape,and radial enhancement(P<0.05).ROC curve results showed that the AUC of serum miR-9-5p and miR-214-5p for diagnosing malignant breast nodules was 0.879 and 0.837,respec-tively.The consistency between CEUS and serum miR-9-5p,miR-214-5p alone or in combination for the diagnosis of malignant nodules and pathological results showed Kappa values of 0.669,0.622,0.553,and 0.857.The specificity,accuracy,and posi-tive predictive value of CEUS combined with serum miR-9-5p and miR-214-5p in distinguishing malignant breast nodules were higher than those of CEUS,miR-9-5p,and miR-214-5p alone(P<0.05).Conclusion The expression level of serum miR-9-5p increases and the expression level of miR-214-5p decreases in patients with malignant breast nodules.CEUS combined with se-rum miR-9-5p and miR-214-5p has high diagnostic value in distinguishing between benign and malignant breast lesions.
Keywords：Breast nodulesContrast-enhanced ultrasoundBenign and malignant nodulesMicroRNA-9-5pMicroRNA-214-5p
Publication Date：2025-11-30
Online Publishing Date：2026-01-09(First online date of this platform, not the publication date of the document)
Pages：5( 67-71 )