Diagnostic value of lung ultrasound score combined with serum lncRNA MALAT1 and lncRNA NEAT1 levels for neo-natal respiratory distress syndrome

YAO Junfang; YAN Yuanyuan; LOU Junge; SHEN Li; YANG Lijuan

Abstract：Objective To investigate and analyze the diagnostic value of lung ultrasound score(LUS)combined with serum long non-coding RNA(lncRNA)metastasis-associated lung adenocarcinoma transcript 1(MALAT1)and nuclear-enriched abun-dant transcript 1(NEAT1)levels for the severity of neonatal respiratory distress syndrome(NRDS).Methods Eighty-two NRDS neonates delivered in our hospital were selected as the NRDS group and were categorized into mild group(41 cases),moderate group(31 cases),and severe group(10 cases)with reference to the Berlin definition.85 healthy premature neonates in our hospital were regarded as the control group.All neonates in the group underwent lung ultrasonography.The expression lev-els of MALAT1 and NEAT1 in serum were detected by real-time fluorescence quantitative PCR.Receiver operating characteristic curve(ROC)was used to evaluate the predictive value of LUS combined with MALAT1 and NEAT1 in neonatal moderate and se-vere NRDS.Spearman was used to analyze the correlation between NEAT1,MALAT1 and LUS.Results Compared with the control group,the left lung,right lung,bilateral lung bases,bilateral lungs,both lungs and the relative expression levels of NEAT1 and MALAT1 were higher in the NRDS group(P<0.05).The LUS and the relative expression levels of serum NEAT1 and MALAT1 in the moderate group were higher than those in the mild group(P<0.05),the LUS and the relative expression lev-els of serum NEAT1 and MALAT1 in the severe group were higher than those in the moderate and mild groups(P<0.05).The area under the curve(AUC)of the LUS combined with MALAT1 and NEAT1 to predict moderate and severe NRDS was 0.969 and 0.965,respectively.LUS was positively correlated with serum NEAT1 and MALAT1 levels(P<0.05).Conclusion LUS and expression levels of NEAT1 and MALAT1 increase with the severity of NRDS in children with NRDS.LUS combined with MALAT1 and NEAT1 has predictive value for moderate and severe NRDS.