Expression of CD226 in preeclamptic placentas and its role in trophoblast function
YU Yang
BAI Jinbo
FENG Dan
XU Xiaoyan
WANG Bo
LIU Zekun
ZHANG Ming
Abstract：Objective To analyze the expression characteristics of CD226 in placental tissues from preeclampsia(PE)patients and its biological function in trophoblast cells.Methods CD226 mRNA transcript levels and protein expression in placental tissues from the PE patients and women with normal pregnancies were detected by qRT-PCR and immunohistochemical staining.In the trophoblast cell lines HTR8/SVneo and JAR,CD226 gene was knocked down or overexpressed,respectively,to evaluate the impact of its expression changes on cell proliferation,apoptosis,migration,and invasion.Western blot analysis was used to detect the effects of CD226 on the protein expression of TNF-α and NF-κB p65.Results Compared with the normal pregnancy group,the expression level of CD226 was significantly up-regulated in the placental tissues of the PE group.Knockdown of CD226 enhanced the proliferation,migration,and invasion,and suppressed apoptosis in HTR8/Svneo cells.Conversely,the overexpression of CD226 significantly inhibited the proliferation,migration,and invasion of JAR cells,while promoting apoptosis in these cells.Furthermore,Western blot analysis revealed that CD226 overexpression promoted the protein expression of TNF-α and NF-κB p65 in trophoblast cells.Conclusion The expression level of CD226 in placental tissues of PE is higher than that in normal pregnancy group.CD226 exerts the inhibitory effects on the proliferation,migration,and invasion,and promotes apoptosis in trophoblast cells.
Keywords：PreeclampsiaCD226Placental tissueProliferationInvasion
Publication Date：2025-12-25
Online Publishing Date：2026-01-16(First online date of this platform, not the publication date of the document)
Pages：8( 1398-1405 )