The effect of combined photodynamic therapy with α-melittin-PPIX-NP on the biological behavior of B16-F10 cells
LI Sanrong
ZHANG Yan
XIAO Min
GENG Yuanyuan
Abstract：Objective To investigate the inhibitory effect of melittin combined with photodynamic therapy(PDT)on the biological behavior of melanoma cells(B16-F10).Methods Nano-drug bee venom peptide photosensitive lipid nanoparticles(α-melittin-PPIX-NP)were prepared and characterized by hydrolysis method.The red blood cell hemolysis experiment verified the hemolytic side effects of melittin.CCK-8 was used to detect its ability to kill B16-F10 cells in combination with PDT.Western Blot was used to detect the expression of SR-B1 receptor protein in different cells.Flow cytometry was used to detect cell apoptosis,drug uptake by different cells,and in vitro activation of bone marrow-derived dendritic cells(BMDCs).Results The prepared α-melittin-PPIN-NP had uniform morphology and concentrated hydrated particle size distribution,which significantly reduced the side effects of erythrocyte hemolysis.α-melittin-PPIX-NP can efficiently target B16-F10 cells and inhibit the proliferation of tumor cells by activating BMDCs,and its effect is better when combined with near-infrared light(NIR).Conclusion PDT with α-melittin-PPIX-NP combined with NIR can promote the apoptosis of B16-F10 cells,activate BMDC,and effectively increase the anti-tumor immune effect.
Keywords：MelittinPhotodynamic therapyMelanomaHemolysis of red blood cellsSR-B1 receptorCell apoptosisBone marrow-derived dendritic cells
Publication Date：2025-09-25
Online Publishing Date：2025-11-10(First online date of this platform, not the publication date of the document)
Pages：9( 1048-1056 )