Effects of KCNN4 on the biological functions of prostate cancer cells
WANG Danqi
XI Shu
CUI Jinlong
WANG Shichun
ZHANG Jia
SHI Minghui
YUAN Shuai
ZENG Xiantao
Abstract：Objective To investigate the biological function of the intermediate-conductance calcium-activated potassium channel(KCNN4)and the inhibitory effect of its small molecule inhibitor,Senicapoc,in prostate cancer.Methods Small interfering RNA(siRNA)-mediated KCNN4 knockdown was performed in DU145 and C4-2 cell lines.Cell counting kit-8(CCK-8)assays,Transwell assays,and wound-healing assays were conducted to evaluate the changes in cell proliferation and migration.The cell lines WPMY-1,22RV-1,C4-2,and DU145 were treated with Senicapoc.The effects of Senicapoc on cell proliferation and migration were assessed using CCK-8 assays,colony formation assays,and wound-healing assays.Results Downregulation of KCNN4 significantly reduced cell viability and cell migration(P＜0.05).Senicapoc treatment significantly decreased cell viability,colony numbers,and migration rates(P＜0.05).Conclusion KCNN4 promotes the proliferation and migration of prostate cancer cells.Targeting KCNN4 may represent a novel therapeutic strategy for prostate cancer.
Keywords：KCNN4Prostate cancerIon channelsProliferationMigration
Publication Date：2025-02-27
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 168-174 )