Application of Adipose Derived Mesenchymal Stem Cells in the Treatment of Chronic Obstructive Pulmonary Disease

doi:10.3969/j.issn.1004—5511.2019.02.012

Abstract：Objective To explore the application value of adipose derived mesenchymal stem cells( ADMSCs) in chronic obstructive pulmonary disease( COPD). Methods 60 SPF male rats were randomly divided into control group,observation group and model group with 20 rats in each group. COPD rat models were established in observation group and model group by passive cigarette smoking and intratracheal instillation of endotoxin,while non model was established in control group. After modeling,rats in observation group were injected with 0. 5 ml of BrdU labeled ADMSCs suspension through caudal vein,while rats in control group and model group were injected with 0. 5 ml of normal saline through caudal vein. Lung function,levels of inflammatory factors and pathological changes of lung tissue in rats were determined at 14 d after transplantation. Results Compared with the control group,the indexes of lung function decreased significantly in model group and observation group(P〈0. 05),and lung function in observation group was better than that in model group(P〈0. 05). The number of total white blood cells,number of macrophages and neutrophils,levels of interleukin 8,tumor necrosis factor and C reactive protein in bronchoalveolar lavage fluid of the model group and observation group were significantly higher than those in control group(P〈0. 05). The number of immune cells and levels of inflammatory factors in observation group were significantly lower than those in model group ( P〈0. 05). Pathological changes of COPD in the lung tissue sections of model group and observation group were ob—served. Emphysema symptoms in observation group were milder than those in model group(P〈0. 05). Conclusion ADMSCs transplantation can significantly improve pathological changes of lung tissues in COPD rats,relieve inflamma—tory reaction and improve lung function.