Changes and Significances of MicroRNA-126 Expression in Directional Differentiation of Pluripotent Stem Cells to Retinal Neural Stem Cells
Abstract：Objective To investigate changes and significances of microRNA-126(miR-126)expression in directional differentiation of pluripotent stem cells(iPSCs)to retinal neural stem cells(RNSCs)in patients with wet age-related macular degeneration(wet AMD).Methods Ten patients with wet AMD were chosen as observation group and ten healthy people were chosen as control group.Peripheral venous blood samples were collected,from which peripheral blood mononuclear cells(PBMCs)were extracted,and CD34+ cells were extracted.CD34+ cells were transfected with adenovirus and iPSC was detected.Relative expression of miR-126 at 10 different times in the process of RNSC were examined by quantitative real-time PCR(qRT-PCR).Results RNSC could be differentiated from peripheral venous blood in patients with wet AMD.There were significant differences on miR-126 expression at different times except time 8,9,10 in observation group(P<0.05);There were no significant differences on miR-126 expression at different times in control group(P>0.05);There were significant differences on miR-126 expression at different times between the two groups(P<0.05).Conclusion The expressions of miR-126 increase in patients with wet AMD and gradually decrease with time.
Keywords：Wet age-related macular degenerationPeripheral blood mononuclear cellPluripotent stem cellRetinal neural stem cellMicroRNA-126
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 130-133 )