Protective Effect of Pretreatment with Panax Notoginseng Saponins and Rb1 on Myocardial Ischemia and Reperfusion Injury in Rats
Abstract：The purpose is to study the effect of panaxtnotoginsengsaponins(PNS) pretreatment or Rb1 on myocardial ischemia/reperfusion injury in rats and the relation among protective effect of PNS or Rb1,heat shock protein 70 and ε-PKC.Forty-two SD rats were dividrd into four group:Normal group(n=6),Control P group(n=12), PNS group(n=12) and Rb1 group(n=12).Malondialdehyde(MDA) and superoxide dismutase (SOD)contents were determined after 40 min ischemia followed 30 min reperfusion 。HSP70 and ε-PKC were detected by immunohistochemical technique after reperfusion of 120 min.The results showed that: As compared with the control,PNS snd Rb1 groups can significantly reduce MDA production and protect SOD activity(P＜0.01).MDA production in PNS group was less than that in Rb1 group(P＜0.01) and SOD activity in PNS group was less than that in Rb1 group(P＜0.05).The HSP70 levels in PNS were higher than that of the control and Rb1 grougs (P＜0.01).Compared with the normal,ε-PKC in PNS group was reduced slightly (P＞0.05).In the control and Rb1 groups ε-PKC were reduced significantly (P＜0.01).The results indicated that PNS can improve the expression of heat shock protein 70 in pretreated rats and has better protective effects than that of Rb1 against myocardial ischemia/reperfusion injury.
Keywords：Psnas notoginseng saponinsMyocardial ischemia/reperfusioninjuryHeat shock protein
Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 33-35 )