Pilot Study on Hepatitis B Virus Core Promoter Mutations in Patients
Abstract：To establish PCR for detection hepatitis B virus (HBV) DNA andDetect hepatitis B virus core promoter (CP) mutations in patients with hepalitis B,polymerase chain reaction amplified HBV DNA fragments were directly sequenced and common-source analyzed with the help of computer. The results showed that the positive rate of serum samples from 20 HB patients was 95%(19/20).The HBV CP fragments of two patients and pGEM.7 Z-HBV plasmid were sequenced. Compared with report gene sequence,the identical rate of 2 patients were only 72.0%,66.5% respectively,but pGEM. 7 Z-HBV plasmid was 90.0%.This study indicated that PCR may be used in the detection of HBV with mutations in CP region and CP region mutation exists in patient with chronic hepatitis B.
Keywords：Hepatitis B virusCore promoterMutation
Publication Date：2001-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )