Mechanism of resveratrol on mitochondrial autophagy,stress response and IL-33/ST2 signaling pathway in rats with periodontitis

LIU Zhenyu; WANG Ye; HUANG Liyun; ZHUO Shuna; SUN Dalei; ZHANG Bohao

doi:10.15956/j.cnki.chin.j.conserv.dent.2026.02.002

Abstract：Objective To explore the mechanism of resveratrol on mitophagy,stress response and interleukin-33/tumorigenic 2 receptor(IL-33/ST2)signaling pathway in periodontitis rats.Methods 54 rats were randomly divided into N group,M group,Z group and L,H group,with 10 rats in N group and 11 rats in every remaining group.Rats in group Z were intragastrically administered with 0.05 g/kg metronidazole aqueous solution,rats in groups L and H were intragastrically administered with 5 and 15 mg/kg resveratrol,respectively,and rats in groups N and M were intragastrically administered with the same dose of normal saline.1 time/d,continuous treatment for 7 d.Periodontal tissue was isolated and the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in periodontal tissue and serum were detected.The levels of osteoprotegerin(OPG)and its ligand(OPGL)in periodontal ligament were detected by RT-PCR.The level of mitophagy was observed by electron microscopy.The morphology of periodontal tissue was observed by HE staining.The expression of IL-33 and ST2 protein was detected by western blot.Results Compared with group N,the levels of MDA,OPGL mRNA,IL-33 and ST2 protein in group M,group Z and group L,H were increased,and the levels of SOD,GSH-Px,OPG mRNA and OPG/OPGL ratio were decreased(P<0.05).Compared with group M,MDA,OPGL mRNA,IL-33 and ST2 in group Z and L,H decreased,and those in group H were the lowest.SOD,GSH-Px,OPG mRNA and OPG/OPGL ratio increased,and those in group H were the highest(P<0.05).Under the electron microscope,the mitochondrial ridge morphology of the Z group and the L,H group tended to be normal,and the autophagosomes and vacuolar changes were reduced,and the mitophagy of the H group was the least.HE staining showed that the periodontal tissue morphology of L group,Z group and H group was improved,and the morphology of H group was the best.Conclusion High dose of resveratrol can improve mitochondrial autophagy,reduce stress response,improve periodontal morphology and inhibit IL-33/ST2 signaling pathway activation in periodontitis rats.