The therapeutic effect of flavonoids form rhizoma drynaria on periodontitis in rats:An experimental study
LIU Xinyue
LI Wenpei
ZHAO Zipeng
ZHANG Xiaoran
MA Zhe
LI Chunnian
Abstract：Objective To investigate the therapeutic effect of the flavonoids from rhizoma drynaria on experimental periodontitis in rats.Methods Forty 2-month-old male SD rats were used,with 10 rats in each group.An experimental periodontitis model was established by ligating the upper first molar with orthodontic ligatures.The rats were randomly divided into four groups:no intervention(Group A),periodontal pocket injection of drug-free hydrogel(Group B),periodontal pocket injection of drug-free hydrogel+oral administration of the flavonoids from rhizoma drynaria(Group C),and periodontal pocket injection of the flavonoids from rhizoma drynaria-loaded hydrogel(Group D).The rats were sacrificed after 4 weeks of medication,and the maxillary bone specimens were taken for HE staining to observe the periodontal pathological tissues,TRAP staining to observe the number of osteoclasts,and immunohistochemistry to detect the expression of bone protection protein(OPG),nuclear factor κB receptor activator ligand(RANKL),and type I collagen(COL1A1)in the periodontal tissues.Finally,the experimental data were statistically analyzed.Results HE staining showed that compared with the non-medication group,local or systemic administration of the flavonoids from rhizoma drynaria could lead to new bone formation,and local administration of the flavonoids from rhizoma drynaria generated more bone-like tissue than systemic administration,with more orderly arrangement of fibrous tissue.TRAP staining revealed that the number of osteoclasts in the non-medication group,systemic medication group,and local medication group showed a decreasing trend,with statistically significant differences(P＜0.05).Immunohistochemistry results showed that the expression of osteogenic-related factors OPG,COL1A1 in the non-medication group,systemic medication group,and local medication group showed an increasing trend,with statistically significant differences(P＜0.05),while the expression of RANKL showed a decreasing trend,with statistically significant differences(P＜0.05).Conclusion The flavonoids from rhizoma drynaria have a therapeutic effect on experimental periodontitis in rats.It can better promote new bone formation,inhibit the number of osteoclasts,and promote the repair of periodontal tissues by increasing the expression of OPG and COL1A1 and reducing the expression of RANKL.From the perspective of medication methods,the effect of local administration of hydrogel-laden medication is better than systemic administration.
Keywords：flavonoids from rhizoma drynariaperiodontitislocal administrationhydrogel sustained-releasebone regenerationOPG/RANKL pathway
Publication Date：2026-01-28
Online Publishing Date：2026-03-11(First online date of this platform, not the publication date of the document)
Pages：8( 2-9 )