Levels and clinical significance of serum CTRP3 and CYTL-1 in patients with chronic periodontitis

LI Shuo-xi; LIU Ying; DONG Yang

doi:10.15956/j.cnki.chin.j.conserv.dent.2025.07.003

Abstract：AIM:To investigate serum levels of C1q/TNF-related protein 3(CTRP3)and cytokine receptor like factor 1(CYTL-1)in patients with chronic periodontitis,and to assess their correlation with periodontal and inflammatory indicators.METHODS:90 patients with chronic periodontitis admitted to our hospital were enrolled as the periodontitis group.Based on the periodontal severity,they were classified into a mild group(33 cases)and a moderate to severe group(57 cases).Another 40 oral health patients who underwent physical examinations at our hospital during the same period were as the healthy control group.The periodontal indicators-including probing depth(PD),bleeding index,plaque index(PLI),attachment loss(AL),and gingival index-were recorded.Serum levels of Interleukin-1β,Interferon-γ(IFN-γ),Tumor necrosis Factor-α(TNF-α),CTRP3,and CYTL-1 were measured by enzyme-linked immunosorbent assay.The correlation between serum CTRP3、CYTL-1 and gingival crevicular fluid and periodontal indicators was analyzed;the value of CTRP3 and CYTL-1 in diagnosing the severity of chronic periodontitis was compared.RESULTS:Compared with the healthy control group,patients with chronic periodontitis had significantly higher serum levels of IL-1β,IFN-γ,and TNF-α,and significantly lower levels of CTRP3 and CYTL-1(P＜0.05).The moderate-to-severe group exhibited higher levels of inflammatory markers and worse periodontal parameters than the mild group(P＜0.05),accompanied by lower CTRP3 and CYTL-1 concentrations(P＜0.05).Serum CTRP3 and CYTL-1 levels were negatively correlated with IL-1β,IFN-γ,TNF-α,PD,BI,PLI,AL,and GI(P＜0.05).Combined detection of CTRP3 and CYTL-1 yielded a significantly greater area under the ROC curve(AUC)for diagnosing disease severity compared to either marker alone(P＜0.05).CONCLUSION:The serum levels of CTRP3 and CYTL-1 are significantly decreased in patients with chronic periodontitis,and are related to periodontal status and systemic inflammation.They can be used to diagnose the severity of chronic periodontitis,and combined detection has more diagnostic value.