The molecular mechanism of salidroside in the treatment of apical periodontitis based on network pharmacology and molecular docking
LIU Han
WANG Ze-cheng
ZHANG Chong-yang
ZHANG Ya-qing
WANG Sheng-chao
YUAN Yi-fang
ZHANG Rong
JIANG Wen-kai
HE Ya-ni
Abstract：AIM:The multi-target mechanism of Salidroside in the treatment of periapical inflammation was systematically revealed by integrating network pharmacology and molecular docking techniques.METHODS:SwissTargetPrediction,SEA and SuperPred multi-platform combined prediction of salidroside action targets;Based on GeneCards,DisGeNET and CTD three databases,the related targets of periapical inflammation were screened.The PPI interaction network was constructed using STRING platform,and topological feature analysis was carried out by Cytoscape 3.10.0 to identify key hub targets.GO/KEGG multi-dimensional functional enrichment analysis was carried out using the"Weisheng"online system,and the target-pathway interaction network was constructed.The core target(PDB ID acquisition)was selected for molecular docking verification through AutoDock4.RESULTS:Target intersection analysis identified 39 key regulatory targets,including DPP4,GAPDH,MMP9 and other core proteins.KEGG pathway enrichment revealed 90 significant signal axes,among which IL-17 and Rap1 signaling pathways showed outstanding regulatory value.Molecular docking verification showed that salidroside had strong binding properties with 6 key targets(binding energy ≤-6.8 kcal/mol).CONCLUSION:This study found that salidroside achieves three-dimensional therapeutic effect of periapical inflammation(immune regulation-inflammation control-tissue regeneration)through multi-target synergistic regulatory mechanism,especially through IL-17 signaling axle-mediated inflammation regulation and Rap1 pathway driven bone repair process.This study provides an innovative mechanism explanation and theoretical basis for the treatment of oral inflammatory diseases by traditional Chinese medicine monomeric.
Keywords：apical periodontitissalidrosidenetwork pharmacologymolecular docking
Publication Date：2025-07-28
Online Publishing Date：2025-08-21(First online date of this platform, not the publication date of the document)
Pages：7( 373-379 )