Construction of dental follicle stem cell/tannic acid complex cell sheet to promote alveolar bone regeneration
JU Rong-bai
LIU Ting-jie
LI Bei
HE Min
Abstract：AIM:This study aimed to investigate the effect of TA(Tannic acid)on the properties of dental follicle stem cell(DFSC)membrane sheets and clarify the potential of TA-containing composite cell membrane sheets in repairing periodontal defects.METHODS:DFSC membrane sheets treated with different concentrations of TA were first prepared.Hematoxylin and eosin(HE)staining was used to examine the membrane sheet structure,immunofluorescence staining was employed to analyze the expression of related proteins,and cell proliferation assays were conducted to measure cell viability.The intracellular ROS level was detected by the fluorescent probe DCFH-DA,the expression of oxidative stress-related factors was explored by RT-qPCR,and the scavenging ability of exogenous ROS was measured by the DPPH method.A periodontal defect model was also established in rats,and the repair effect was evaluated by Micro CT,HE and Masson staining.RESULTS:The medium concentration TA group exhibited significant performance in maintaining cell stemness,inducing osteogenic differentiation and in vivo periodontal tissue regeneration,while the low concentration was beneficial for cell proliferation.CONCLUSION:TA-composited DFSC membrane sheets can effectively repair periodontal defects in rats,indicating that the composite membrane sheets possess have potential in periodontal defect repair.
Keywords：tannic acidcell sheetdental follicle stem cellsperiodontal defects
Publication Date：2025-03-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 132-138,146 )