The effects of estrogen on mandibular condylar chondrocytes via HIF2α
NIU Lin-sheng
YE Tao
SUN Dong-liang
ZHANG Mian
MIAO Hui
YU Shi-bin
Abstract：AIM:Toinvestigate the effects of estrogen on mandibular condylar chondrocytes via HIF2α. METHODS:The mandibular condylar chondrocytes from the second passage were divided into control group,estrogen (1 μmol/L)-stimulating group,estrogen-stimulating group,estrogen receptor blocking group and estrogen-stimula-ting and HIF2α blocking group. The cells were collected after being stimulated for 12 hours. Real-time PCR and western blot were employed to detect the expression of the main cartilage matrix aggrecan(Acan),collagen type Ⅱ(Col Ⅱ),proinflammatory cytokines interleukin-1β (IL-1β),tumor nectosis factor-α (TNF-α),HIF2α,HIF2α downstream cytokines matrix metalloproteinase 13 (MMP13),vascular endothecial growth factor (VEGF)and collagen type X (Col X). RESULTS:Stimulation with estrogen at 1 μmol/L significantly decreased the expression of Acan and Col Ⅱ(P＜0.05),but increased the expression of IL-1β,TNF-α,HIF2α,MMP13,VEGF and Col X(P＜0.05). Both specific estrogen receptor blocking agent and specific HIF2α blocking agent reversed the related effect of estrogen (P＜0.05). CONCLUSION:Estrogen can induce degenerative changes of mandibular condylar chondrocytes. The pathway of estrogen-estrogen receptor-HIF2α plays an important role in this process.
Keywords：estrogenhypoxia inducible factortemporomandibular jointcondylechondrocyte
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 321-326,371 )