An investigation on antibacterial effects of Nd:YAG laser on the Porphyromonas gingivalis
WU Yi-fan
SONG Zhong-chen
SHU Rong
Abstract：AIM:To investigate the antibacterial effects of Nd:YAG laser against Porphyromonas gingivalis ( P. gingivalis) in vitro. METHODS:P. gingivalis were in vitro cultured and P. gingivalis biofilm was prepared. The cultured P. gingivalis were treated by Nd:YAG laser at 6 W for 0～60 s respectively. The biofilm formation ability of planktonic P. gingivalis was observed by crystafl violet test; the viability of P. gingivalis cells in mature biofilms was detected by confocal Laser scanning microscopy ( CLSM);the morphology of mature biofilms was observed by scanning electron microscopy ( SEM) . RESULTS:The biofilm formation ability of P. gingivalis was limted under 35 s laser radi-ation. The viability of P. gingivalis in mature biofilm went down under 45 s iradiation. The deformation of mature bio-film and the deconstruction of becterium became distincted with the iradiation time increased. The major destructional form was evaporation. CONCLUSION:Nd:YAG laser may inhibit of biofilm formation of planktonic P. gingivalis and destroy the construction of the mature biofilm and bacteria.
Keywords：periodontal pathogenssingle bacteria biofilmNd:YAG laserin vitro
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 258-264,302 )