The antibacterial effects of silver bromide/cationic polymer nanocomposite-modified glass ionomer cements

HUANG Fang; CHEN Yin-yan; ZHANG Yu; HU Yun-tong; SHEN Li-juan; XIAO Yu-hong

doi:10.15956/j.cnki.chin.j.conserv.dent.2018.03.003

Abstract：AIM: To investigate the antibacterial activity and cytotoxicity of glass ionomer cement (GIC) supplemented with a silver bromide/cationic polymer nanocomposite (AgBr/BHPVP ). METHODS:AgBr/BHPVP were incorporated into the GIC at 3%,4% and 5% (w/w) respectively(test groups). A conventional GIC,FujiⅡcement was used as the control. The antibacterial activity of the materials on Streptococcus mutans was evaluated by agar- diffusion test on days 1,7 and 14 respectively. The biofilm staining of live and dead bacteria on the surface of the specimens was observed by confocal laser scanning microscope(CLSM). The cytotoxicity of the eluent of the sam-ples to human dental pulp cells(hDPCs) was determined by CCK-8 assay. RESULTS:The highest inhibition zone of was found in the test goup of Fuji Ⅱ with AgBr/BHPVP mass fraction of 5%. The inhibition zone increased with the addition of AgBr/BHPVP(P<0.05),and the antimicrobial activity did not decrease with time(P>0.05). CLSM in-dicated that the live bacteria on the specimens of the test groups were significantly fewer than those of control group. Cytotoxicity assay showed that there was no significant difference of cell proliferation between the goup of GIC-contai-ning 3% (w/w) AgBr/BHPVP nanoparticles and the control group or the blank control group(P>0.05). But in the goup of GIC-containing 4% and 5% (w/w) AgBr/BHPVP nanoparticles the relative cell proliferation was (84.4 ± 0.8)% and (80.8 ± 1)% respectively(vs control,P<0.05). CONCLUSION:The addition of AgBr/BHPVP into FujiⅡGIC may increase antimicrobial properties over the conventional GIC alone for Streptococcus mutants. The cyto-toxicity of GIC contiaining 4% and 5% AgBr/BHPVP to HDPCs is grad Ⅰ.