The effects TNF-α on the proliferation and osteogenic differentiation of periodontal ligament stem cells
MENG Chao-long
WANG Xiang
DUAN Jan-min
LI Hong-tao
LYU Dao-zhi
Abstract：AIM:To investigate the effects of tumor necrosis factor-α(TNF-α) with different concentra-tions on the proliferation and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs) in vitro. METHODS:hPDLSCs were isolated and cultured by limited dilution method. Surface antigens of hPDLSCs were de-tected by flow cytometer.hPDLSCs were treated with TNF-α at 0 ng/ mL(the control group),0.1 ng/ mL,1 ng/ mL, 10 ng/ mL and 50 ng/ mL respectively. Cell proliferation was evaluated using CCK8 assay. hPDLSCs were induced by osteogenic induction medium with 0 ng/ mL,0.1 ng/ mL and 10 ng/ mL TNF-α respectively for consecutive 7 d and 21 d. By means of real-time PCR,the expression Runx2,Osterix,OPN and Bone BSP were measured. Mineral nod-ule formation was evaluated by alizarin red S staining. RESULTS: The cultured hPDLSCs were positive for CD105(96.6%),CD90 (97.1%) and negative for CD34 (1.89%),CD45 (1.65%). TNF-α at 0.1~10 ng/ mL had no influence on cell proliferation, whereas 50 ng/ mL TNF-α inhibited cell proliferation. After osteogenic induction for 7 d,the expression of Runx2 and Osterix in 0.1 ng/ mL TNF-α group was higher than that in control group,whereas in 10 ng/ mL TNF-α group was lower. After osteogenic induction for 21 d,the Mrna levels of Runx2,Osterix,OPN and BSP were increased in 0.1 ng/ mL TNF-α group and decreased in 10 ng/ mL group. Moreover, mineral nodule in 0.1 ng/ mL TNF-α group was more than that in control group and 10 ng/ mL TNF-α group. CONCLUSION:TNF-α at high concentration my inhibit the proliferation at low concentration may promote osteogenic differentiation of Hp-DLSCs.
Keywords：tumor necrosis factor-α(TNF-α)human periodontal ligament stem cells(hPDLSCs)prolifera-tionosteogenicdifferentiation
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 63-68 )