Effects of mechanical centrifugal stress on the proliferation and mineralization of periosteal cells and bone marrow mesenchymal stem cells in vitro
LI Xiao-liang
LIN Cheng
WANG Qiao-yun
LI Ya-mei
BAO Fei
AI Juan
DIAO Zhao-feng
Abstract：AIM:To investigate the proliferation and mineralization of osteoblasts from rabbit periosteal cells (PCs) and bone marrow mesenchymal stem cells(BMSCs) treated by cyclic mechanical centrifugal stress. METH-ODS:Rabbit skull periosteum cells,and femoral BMSCs were in vitro culture,spread to the third passages,and then divided into experimental and control groups. Low-speed centrifuge was used for loading centrifugal force of 170 g, 210 g, and 250 g respectively given to the experimental cells for 10 min/d stress stimulation. The control group cells was placed in the clean bench 10 min/d without centrifugal treatment. All the cells were conventionally cultured for 1, 3,5,7 and 9 d respectively. The cell proliferation and,ALP activity and,mineralization were examined,and the rela-tive expression levels of OCN and RUNX2 mRNA were measured by qRT-PCR. RESULTS:Cyclic mechanical cen-trifugal stimulation incveased the proliferation activity,ALP activity and the number of mineralized nodules of PCs and BMSCs,and the number of mineralized nodules in 2 weeks after loading was significantly increased. The relative ex-pression of osteocalcin OCN and RUNX2 mRNA was significantly increased. The growth of PCs in 250 g group was the most significant(P<0.05). CONCLUSION: Periodic mechanical centrifugal stimulation can promote the prolifera-tion and of rabbit PCs and BMSCs in vitro.
Keywords：periosteal cellsbone marrow-derived mesenchymal stem cellsperiodic mechanical centrifugal forcemineralizationqRT-PCR
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 6-13 )