A study on the differentiation of dental pulp stem cells into odontoblasts by gel with gradient elastic modulus
SU Xiao-ying
LI Zhang-yi
HUANG Jian-yong
YAO Rui
LIU Xiao-zhi
Abstract：AIM:To study the differentiation of dental pulp stem cells (DPSCs) into odontoblasts induced by gel with gradient elastic modulus.METHODS:Mouse DPSCs of passage 4 were seeded on the gel surface with 4 gradient elastic modulus respectively:less than 25 kPa,25 ～ 50 kPa,50 ～ 75 kPa and 75 ～ 100 kPa,which were prepared by a mixture of low melting point agarose and modified Eagle medium,and cultoured for 21 d.The mineralized nodules in the culture were detected using alizarin red staining,and the mRNA and protein expression of dentin matrix protein 1 (DMP1),dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) was tested by reverse transcription polymerase chain reaction and western blot method respectively.RESULTS:The cultured cells expressed CD29 and CD90,almost did not express CD34 and CD45.With the increase of elastic modulus of the solid gel,the mineralized nodule in the culture increased,and mRNA and protein expression level of DMP1,DSP and DPP also increased.CONCLUSION:DPSCs may differentiate into odontoblasts when seeded on the gel surface with different elastic modulus in a gradient dependent form.
Keywords：dental pulp stem cellsdentinelastic modulusdifferentiation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 629-633,628 )