Experimental study on the effect of siRNA interference on the development of periodontitis in animals
WANG Yang
WANG Mu-yang
CUI Yi
FEI Dong-dong
WANG Qin-tao
LIN Ying
Abstract：AIM:To study the silence of Casein kinase 2-interacting protein 1 (CKIP-1) gene by siRNA interference on the development of periodontitis in rats.METHODS:9 SD rats were randomly divided into 3 groups (n =3).The rats in group NC and group PC were treated by ligation and injection of LPS-PG at second maxillary molars for the establishement of periodontitis modelse.During the construction of the model,the rats in PC group were injected with CKIP-1 siRNA,and in NC group with saline as a control.The rats without treatment were used as the blank controls(BC goup).20 and 30 d after set-up of the experement Micro-CT and HE staining was performed to campare the damage of alveolar bone and the inflammatory response of the rats among the groups.RESULTS:The decrement of alveolar bone height in NC group was much more than that in PC group,alveolar bone absorption (mm):0.9606 ± 0.1112 vs 0.6759 ± 0.0514;alveolar bone volume percentage (％):0.5958 ± 0.014 vs 0.7509 ± 0.0355 (P ＜ 0.05).The alveolar bone absorption and the periodontal fiber sorting disorder was more obvious in the NC group than in the PC group.CONCLUSION:Silencing of CKIP-1 gene by siRNA interference can reduce the bone loss and inflammation in the rats with periodontitis.
Keywords：siRNA interferenceCKIP-1 geneperiodontitisrats
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 563-568 )