The effects of human umbilical cord mesenchymal stem cells modified microenvironment on the pluripotency of human dental pulp cells in vitro
HUANG Hao-quan
LIU Lu
XU Zhe-zhen
WEI Xi
Abstract：AIM:To investigate the effects of human umbilical cord mesenchymal stem cells modified microenvironnent (UM) on the pluripotency of human dental pulp cells (DPCs) in vitro.METHODS:DPCs were cultured in UM via an indirect co-culture system consist of UCMSCs and DPCs established by using transwell.DPCs proliferation,senescence,cell cycle distribution and pluripotent factors were detected by CCK-8,flow cytometry analysis,SA-β-gal staining,RT-PCR and western blot.Tri-lineage differentiation potential was compared by specifically staining quantitative analysis and RT-PCR respectively.To measure the content of bFGF secreted by UCMSCs,ELISA method was used.bFGF and SU5402 (FGF receptor inhibitor) were used respectively or in combination to prove the effects of UM and bFGF on DPCs.RESULTS:Proliferation,the level of mRNA and proteins related to pluripotentcy and the ability of multi-differentiation of DPCs were enhanced and the senescence of DPCs was depressed by UM and bFGF respectively or in combination.Except for the chondrogenic differentiation,SU5402 restrained the enhancement of UM on DPCs,but boosted cell senescence.CONCLUSION:UM promotes the pluripotency of DPCs mainly through bFGF secreted by UCMSCs.
Keywords：dental pulp cellsumbilical cord mesenchymal stem cellsmicroenvironmentbasic fibroblast growth factorpluripotency
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 555-562 )