The effects of additional fluoride on the osmotic pressure of DMEM and its influence on the morphology of ameloblasts

ZHANG Le; CAO Wei-jing; GAO Jiang-hong; DONG Ning; RUAN Jian-ping

doi:10.15956/j.cnki.chin.j.conserv.dent.2017.09.003

Abstract：AIM:To study the effects of additional fluoride on the osmotic pressure of DMEM and its influence on the morphology of ameloblasts.METHODS:The osmotic pressure of DMEM containing 10％ fetal bovine serum and NaF at 0(control),10.5,21,42,63 and 84 mg/L respectively was measured.Three groups of DMEM containing NaF of 0,42 and 84 mg/L,six sets of DMEM without NaF and with additional 58.5 mg/L of NaCl,0.05 mg/L of glycerol,1 mg/mL of sorbitol and with the same penetrating hydraulic data as with that of 42 mg/L NaF;and with 117 mg/L of NaCl,0.09 mg/L of glycerol,1.5 mg/mL of sorbitol and with the same penetrating hydraulic data as with that of 84 mg/L of NaF,respectively,were prepared for the experiments.Ameloblasts were cultured in the prepared the medium for 48 h.The morphology of the cells was respectively observed by HE staining and inverted microscope.RESULTS:The osmotic pressure of DMEM with additional NaF ＜ 42 mg/L was lower than than that of the control,The osmotic pressure of DMEM with additional NaF≥42 mg/L was higher than that of the control.T.In the 42 mg/L NaF group,a few cells were wrinkled,dwindled and suspended.In the 84 mg/L NaF group almost all the cells suspended and only a few cells attached,and whose cytomembrane were broken.The cellular morphology in NaCl group,glycerol group and sorbitol group were similar to that of the control group.HE staining showed that in 42 mg/L NaF group,most of the cells had vacuolation.In 84 mg/L NaF group,few cells were attached and wrinkled and dwindled into a mass.The ameloblasts in other groups were similar with those in the control group.CONCLUSION:The osmotic pressure of DMEM varys with the different concentration NaF.The osmotic pressure change of medium did not influnce ameloblasts morphology.