HMGB1 induced by mechanical stress promotes the migration of human periodontal ligament cells through regulating matrix metalloproteinases
YU Xiao-qin
GAO Jin-yu
LIU Hua
Abstract：AIM:To study the role and mechanism of high mobility group box protein 1 (HMGB1) in mechanical stress induced the migration of human periodontal ligament cells (hPDLCs).METHODS:The expression of HMGB1 and matrix metalloproteinase 2/9 (MMP2/9) was assayed by real-time PCR and western blotting when cells were cultured with mechanism stress with 10％ deformation,and 0.5 Hz sinusoidal wave for 0,6,12,24 and 48 h respectively.Then,HMGB1 expression was downregulated by siRNA,the migration of hPDLCs was examined by transwell,MMPs expression was analyzed by real-time PCR,and the levels of ERK1/2 and p-ERK1/2 were determined by western blotting.Additionally,hPDLCs were pretreated with 1 μmol/L of Batimastat (a MMPs inhibitor) and 50 nmol/L of GDC-0994 (an ERK1/2 inhibitor) respectively for 2 h and then treated with mechanism stress for 24 h,the cell migration and MMPs expression was detected,respectively.RESULTS:The mRNA and protein expression of HMGB1,MMP2 and MMP9 were all increased at 6,12 and 24 h in a time-dependent manner,while reduced at 48 h.Downregulation of HMGB1 inhibited mechanical stress-induced the migration of hPDLCs and the expression of MMP2,MMP9 and p-ERK1/2.Batimastat suppressed hPDLCs migration.The expression of MMP2 and MMP9 induced by mechanical stress was dampened by GDC-0994.CONCLUSION:HMGB1 mediates mechanical stress-induced the migration of hPDLCs,which may be through activating ERK1/2 signaling and upregulating MMPs expression.
Keywords：HMGB1mechanical stresshuman periodontal ligament cellsmigrationmatrix metalloproteinase
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 499-505 )