Evolution analysis of cytolethal distending toxin in A.actinomycetemcomitans
SUN Jing-hua
ZHANG Chen
YU Gang
HOU Ben-xiang
Abstract：AIM:To predict sequence variation of cytolethal distending toxin(CDT) subunits of A.actinomycetemcomitans (Aa)in different bacteria using bioinformatics methods.METHODS:The ncbi genbank database,blastP,tblastn and maximum likelihood for phylogenetic tree construction in MEGA were used to study sequence variation of 3 CDT subunits in bacteria.RESULTS:27 species in 20 genus containing CDT subunits in 674 bacterial genus were found in genbank database,and 11 species only had CdtB in their genome.CdtB showed more protein sequence conservation than other 2 subunits in CDT,and sequence variation in CdtB is not correlated with other two CDT subunits in bacteria in evolution.Phylogenetic analysis showed that the CdtB sequence in Aa was more closer to species in Helicobacter,Salmonella and Helicobacter genus than other bacteria.CONCLUSION:Sequence evolution in CdtB protein is significantly different with that in other 2 subunits in CDT complex in Aa.A lot of bacteria species only have CdtB in their genome,and the function of CDT in these bacteria may be acted by CdtB alone without other 2 subunits.
Keywords：A.actinomycetemcomitans (Aa)cytolethal distending toxin (CDT)evolutionbioinformatics
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 493-498,535 )